KMID : 0861020160310010077
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Korea Journal of Herbology 2016 Volume.31 No. 1 p.77 ~ p.82
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Effects of Polygalae Radix Preparata Cum Glycyrrhizae Radix on 4-HNE-induced
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Ham Mee-Jin
Kim Youn-Sub
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Abstract
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Objectives : The study was designed to observe the effect of Polygalae Radix Preparata Cum Glycyrrhizae Radix on 4-Hydroxynonenal (4-HNE)-induced apoptosis in PC-12 cell.
Methods : A cytotoxic test on Polygalae Radix Preparata Cum Glycyrrhizae Radix (PG) was conducted and another MTT assay was conducted to observe the cytoprotective effect against 4-HNE that cause oxidative stress. In addition, in order to observe the expression of Bax, Bcl-2, Caspase-3 and TNF-¥á protein involved with apoptosis, western blot was conducted.
Results : The groups treated with 25 §¶, 50 §¶ and 100 §¶ of PG water extract had no toxicity for PC-12 cell. The groups treated with 25 §¶, 50 §¶ and 100 §¶ of PG water extract showed a significant increase of cell survival rate in comparison with the control group injected by only 4-HNE. The groups treated with 25 §¶ and 50 §¶ of PG water extract showed a significant supression on increase of Bax protein expression in the control group. The group treated with 100 §¶ of PG water extract showed a significant promotion on decrease of Bcl-2 protein expression in the control group. The group treated with 50 §¶ of PG water extract showed a significant supression on increase of Caspase-3 protein expression in the control group. The group treated with 25 §¶ of PG water extract showed a significant supression on increase of TNF-¥á protein expression in the control group.
Conclusions : These results suggest that Polygalae Radix Preparata Cum Glycyrrhizae Radix is effective in reducing apoptosis by 4-HNE-dameged cell.
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KEYWORD
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Polygalae Radix Preparata Cum Glycyrrhizae Radix, Apoptosis, Bax, Bcl-2, Caspase-3, TNF-¥á
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